BIOTECHNOLOGY
Picture Credit: Molly Darlington
Identification of Western Corn Rootworm Chemoreceptors
Chemoperception mechanisms are species-specific among animals, representing a potential field for target identification for pest management. Several studies have described the attraction effect of different exudates and volatiles from corn roots in WCR, including CO2 as the primary attractant, ethylene, β-caryophyllene, and benzoxazinoid complexes. However, the genetic mechanism of host finding is still understudied in this insect.
The goal of this research is to identify and characterize chemosensory genes in Western Corn Rootworm. Results from this research will provide foundational knowledge to species-specific chemoreceptor genes involved in WCR attraction to corn roots to be explored as potential targets for pest management.
Student: Mariana Sanchez Londoño
Susceptibility of Western Corn Rootworm Adult Field Populations to RNAi
The first transgenic RNAi corn expressing the lethal dsRNA dvSnf7 for Western Corn Rootworm management is projected to be released by the end of this decade. Preliminary studies suggest that the effectiveness of RNAi could vary among field populations depending on their physiological and genetic backgrounds. Estimating the level of intra and inter-population variability in susceptibility is essential for detecting field-relevant changes in susceptibility.
The goal of this research is to evaluate the susceptibility of Western Corn Rootworm adult field populations to dvSnf7. This information is essential to determine if corn events expressing dvSnf7 could influence selection pressure in adults affecting resistance evolution.
Student: Matthew Welter
Characterization of RNAi Mode of Action in Western Corn Rootworm
Long-term sustainability of RNAi as a pest management strategy for Western Corn Rootworm through resistance management requires an understanding of the mechanisms involved with dsRNA uptake, processing, and transport.
The goal of this research is to elucidate the pathway(s) and genes involved in RNAi uptake, processing, and spread of dsRNA in Western Corn Rootworm. The information obtained through this research will expand our understanding of the mechanism of action of RNAi, identify potential mechanisms of resistance, and provide insights to improve the efficiency and specificity in both Western Corn Rootworm, insects with recalcitrant RNAi, and aid to increase specificity to the target pest while avoiding effects on non-target species.
Student: Molly Darlington